Natural potential neuroinflammatory inhibitors from Alhagi sparsifolia Shap.

摘要: Neuroinflammation is a key contributor to neuronal damage in neurodegenerative diseases. In our previous work on natural effective neuroinflammatory inhibitors, Alhagi sparsifolia Shap. (Leguminosae), folk medicine widely distributed Xinjiang, attracted attention because of its significant anti-neuroinflammatory effect. Therefore, further investigation the bioactive material basis was carried out. As result, 33 major components were characterized and identified by chromatographic spectral methods, respectively. Furthermore, effects extract purified constituents evaluated LPS-induced N9 cells vitro. The results displayed that compounds 1, 2, 3, 5, 6, 8, 11, 15, 16, 17, 22, 23, 25, 26, 28, 30, could exhibit inhibitory activities without obvious cytotoxicities at their concentrations. Especially, isorhamnetin (1) (IC50 17.87μM), quercetin (2) (10.22μM), 3',7-dihydroxyl-4'-methoxylisoflavone (5) (17.43μM), 3',7-dihydroxyl-4',6-dimethoxylisoflavone (6) (11.21μM), syringgaresinol (16) (2.68μM), bombasinol A (17) (7.61μM), aurantiamide (23) (14.91μM) 1,3,3,4-tetramethyl cyclopentene (33) (2.63μM) showed much stronger inhibiting effect than positive control minocycline (19.89μM). compositions might be responsible for neuroinflammation exhibited herb. Moreover, 16 good leading development potential therapeutic agents against