Changes in Carbohydrate Metabolism in Plasmopara viticola-Infected Grapevine Leaves

摘要: The oomycete Plasmopara viticola is responsible for downy mildew, a severe grapevine disease. In infected leaves, we have observed an abnormal starch accumulation at the end of dark period, suggesting modifications in metabolism. Therefore, several complementary approaches, including transcriptomic analyses, measurements enzyme activities, and sugar quantification, were performed order to investigate understand effects P. infection on leaf and-to larger extent-carbohydrate Our results indicate that associated with increase ADP-glucose pyrophosphorylase (AGPase) activity degradation pathway, especially increased α-amylase activity. Together these alterations metabolism, hexoses, invertase activity, reduction photosynthesis, indicating source-to-sink transition tissue. Additionally, measured disaccharide trehalose correlated trehalase gene expression Altogether, highlight dramatic alteration carbohydrate metabolism later stages development leaves.