Preanalytical Stability of Piperacillin, Tazobactam, Meropenem, and Ceftazidime in Plasma and Whole Blood Using Liquid Chromatography-Tandem Mass Spectrometry.

摘要: BACKGROUND Therapeutic drug monitoring (TDM) is increasingly used to optimize the dosing of beta-lactam antibiotics in critically ill patients. However, beta-lactams are inherently unstable and degrade over time. Hence, patient samples need be appropriately handled stored before analysis generate valid results for TDM. The appropriate handling storage conditions not established, with few conflicting studies on stability clinical samples. aim this study was assess preanalytical piperacillin, tazobactam, meropenem, ceftazidime human plasma whole blood using a liquid chromatography-tandem mass spectrometry method simultaneous quantification. METHODS A reverse phase quantification after protein precipitation developed validated. these assessed EDTA- citrate-anticoagulated at 24, 4, -20°C. analytes EDTA-anticoagulated tubes 24°C. Stability determined by nonlinear regression defined lower limit 95th confidence interval time 15% degradation. RESULTS Based degradation, were stable least 6 hours 24°C, 3 days 4°C, 4 similar. similar CONCLUSIONS Plasma TDM should processed within if kept room temperature 4°C. All long-term -80°C.