Two different types of intervening sequences in the glucoamylase gene from Aspergillus niger.
作者： E. Boel , M.T. Hansen , I. Hjort , I. Høegh , N.P. Fiil
DOI: 10.1002/J.1460-2075.1984.TB02014.X
关键词:
摘要: One single glucoamylase gene could be identified in the chromosomal DNA of Aspergillus niger by Southern blot analysis. This was isolated from a genomic library A. DNA. The is situated on 2.5-kb EcoRI-EcoRV fragment and contains five intervening sequences coding region. 169-bp intron involved differential mRNA processing leading to two different enzymes G1 G2; other four introns are all very small ranging 55 75 bp length. has significant homology region which immediately follows, it internal conserved sequence TACTAAC, also found yeast introns, thought participate splicing. Two transcription initiation sites typical eukaryotic promoter with TATAAT CAAT boxes located upstream gene.
sci-hub.se 参考文章(21)
J L Bennetzen, B D Hall, The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase. Journal of Biological Chemistry. ,vol. 257, pp. 3018- 3025 ,(1982) , 10.1016/S0021-9258(19)81067-0
H. Zalkin, C. Yanofsky, Yeast gene TRP5: structure, function, regulation. Journal of Biological Chemistry. ,vol. 257, pp. 1491- 1500 ,(1982) , 10.1016/S0021-9258(19)68220-7
John H. Pazur, Tadahiko Ando, The action of an amyloglucosidase of Aspergillus niger on starch and malto-oligosaccharides. Journal of Biological Chemistry. ,vol. 234, pp. 1966- 1970 ,(1959) , 10.1016/S0021-9258(18)69850-3
Allan M. Maxam, Walter Gilbert, [57] Sequencing end-labeled DNA with base-specific chemical cleavages Nucleic Acids Part I. ,vol. 65, pp. 499- 560 ,(1980) , 10.1016/S0076-6879(80)65059-9
Stephen M. Mount, A catalogue of splice junction sequences Nucleic Acids Research. ,vol. 10, pp. 459- 472 ,(1982) , 10.1093/NAR/10.2.459
R Breathnach, P Chambon, Organization and Expression of Eucaryotic Split Genes Coding for Proteins Annual Review of Biochemistry. ,vol. 50, pp. 349- 383 ,(1981) , 10.1146/ANNUREV.BI.50.070181.002025
Malcolm J. Casadaban, Stanley N. Cohen, Analysis of gene control signals by DNA fusion and cloning in Escherichia coli Journal of Molecular Biology. ,vol. 138, pp. 179- 207 ,(1980) , 10.1016/0022-2836(80)90283-1
Claudio W. Pikielny, John L. Teem, Michael Rosbash, Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: Implications for U1 RNA and metazoan mRNA splicing Cell. ,vol. 34, pp. 395- 403 ,(1983) , 10.1016/0092-8674(83)90373-2
Kenneth S. Zaret, Fred Sherman, DNA sequence required for efficient transcription termination in yeast Cell. ,vol. 28, pp. 563- 573 ,(1982) , 10.1016/0092-8674(82)90211-2
Jo Ann Wise, David Tollervey, Daniel Maloney, Harold Swerdlow, Eve J. Dunn, Christine Guthrie, Yeast contains small nuclear RNAs encoded by single copy genes Cell. ,vol. 35, pp. 743- 751 ,(1983) , 10.1016/0092-8674(83)90107-1