A comparative evaluation of ileo-rectal anastomosis techniques for the measurement of apparent precaecal digestibilities of folate, niacin and pantothenic acid

摘要: Introduction  There is a great need for better understanding of the intestinal bioavailability B-vitamins and factors that influence it. In food products, however, nutrient content does not necessarily imply proportion actually absorbed. Thus variety experimental approaches have been used determining vitamins. They include measuring changes in tissue vitamin levels, urinary excretion growth response to known intakes vitamin-containing foods (e.g. Southern Baker 1981; Gregory Litherland 1986; Abad 1987; Clifford et al. 1990; Swatilo Yu Kies 1993; Matte Girard 1994). Potential limitations those animal bioassays centre mainly on role microflora extent enhancement or inhibition their synthesis by dietary components. The endogenous flora, with possible uptake distal part small and/or large intestine, complicating factor nearly all B-vitamins. components test diets would stimulate microorganisms could therefore cause overestimation availability conventional bioassays. Application in-vitro assays has also reported water-soluble vitamins, but these are recommended because extrapolation results situation vivo (Holler 1975; Seyoum Selhub 1998). principle, methods using stable isotopes will give most reliable information concerning absorption, application still limited, commercially available specialized facilities expertise required. Ileo-rectal anastomosis proposed as an alternative method calculating nutrients (Herrmann 1988; Green Kiener 1989; Laplace Hennig Roth-Maier important advantage ileo-rectal digestibility prevention coprophagy, digesta can be collected quantitatively via anus. this respect two different techniques developed pigs: end-to-side (ESV) end-to-end (EEV) preserved ileo-caeco-colic valve. Both already compared use vitamins including thiamin, riboflavin B6 (Roth-Maier objective present study was compare both ESV EEV regard values folate, niacin pantothenic acid, which tested hitherto. addition, order examine age- time-related effects periods after surgery were chosen measurement.