Profiling Mycoplasma hyopneumoniae in farms using serology and a nested PCR technique

作者: Carlos Pijoan , Gary J. Bosch , Maria Calsamiglia

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摘要: Implications: The nested PCR detects M. hyopneumoniae earlier then serology. Moreover, detecting from nasal swabs offers new and valuable information in helping interpret serum profiles figuring out when animals get infected how where is being transmitted. This will potentially allow for better timing the application of medication, vaccination, or management strategies.

参考文章(23)
T Young, C H Armstrong, M J Freeman, L Sands-Freeman, M Lopez-Osuna, L J Runnels, Comparison of the enzyme-linked immunosorbent assay and the indirect hemagglutination and complement fixation tests for detecting antibodies to Mycoplasma hyopneumoniae. Canadian journal of comparative medicine. ,vol. 47, pp. 464- 470 ,(1983)
C H Armstrong, M J Freeman, L Sands-Freeman, Serological, pathological and cultural evaluations of swine infected experimentally with Mycoplasma flocculare. Canadian Journal of Veterinary Research-revue Canadienne De Recherche Veterinaire. ,vol. 51, pp. 185- 188 ,(1987)
M. Kobisch, B. Blanchard, M. F. Le Potier, Mycoplasma hyopneumoniae infection in pigs: duration of the disease and resistance to reinfection. Veterinary Research. ,vol. 24, pp. 67- 77 ,(1993)
S Kazama, K Seto, T Yagihashi, Preparation of Mycoplasma hyopneumoniae antigen for the enzyme-linked immunosorbent assay. Canadian Journal of Veterinary Research-revue Canadienne De Recherche Veterinaire. ,vol. 53, pp. 176- 181 ,(1989)
P. S. Paul, R. F. Ross, S. Messier, Humoral and cellular immune responses of pigs inoculated with Mycoplasma hyopneumoniae. American Journal of Veterinary Research. ,vol. 51, pp. 52- 58 ,(1990)
W. Freese, Serology: an aid in the diagnosis of certain respiratory diseases. Agri-Practice. ,vol. 11, pp. 12- 15 ,(1990)
J G Mattsson, K Bergström, P Wallgren, K E Johansson, Detection of Mycoplasma hyopneumoniae in nose swabs from pigs by in vitro amplification of the 16S rRNA gene. Journal of Clinical Microbiology. ,vol. 33, pp. 893- 897 ,(1995) , 10.1128/JCM.33.4.893-897.1995
J. Nicolet, P. Paroz, S. Bruggmann, Tween 20 soluble proteins of Mycoplasma hyopneumoniae as antigen for an enzyme linked immunosorbent assay. Research in Veterinary Science. ,vol. 29, pp. 305- 309 ,(1980) , 10.1016/S0034-5288(18)32632-8
Ryô Harasawa, Kaoru Koshimizu, Osamu Takeda, Takashi Uemori, Kiyozo Asada, Ikunoshin Kato, Detection of Mycoplasma hyopneumoniae DNA by the polymerase chain reaction Molecular and Cellular Probes. ,vol. 5, pp. 103- 109 ,(1991) , 10.1016/0890-8508(91)90003-3