Facile in situ formation of hybrid gels for direct-forming tissue engineering

摘要: Development of bioactive hydrogel as extracellular matrix (ECM) is a very important field for cell-based therapy. In this study, we provided facile method based on sol-gel process fabricating composite hydrogels. The hydrogels were composed derived silica and biopolymer. Different amounts solution (20-80wt%) mixed with 2% polymer sol (alginate) followed by aging gelation to form network so that the alginate-silica hybrid mixture could gel without any additional crosslinking process. self-gelation time measured rheometer was reduced content increased. Such had highly porous interconnected structures. Their strut showed uniform surface texture. Under physiological conditions (PBS, 37°C), these exhibited long-term stability compared alginate control. mechanical properties such compressive strength, modulus, work fracture significantly enhanced hybridization silica. vitro cell tests revealed improved adhesion proliferation behaviors pure cross-linked CaCl2 solution. Furthermore, encapsulation within their suitable microenvironment survival.