Expression and regulation of protein kinase CK2 during the cell cycle

摘要: There are indications from genetic, biochemical and cell biological studies that protein kinase CK2 (formerly casein II) has a variety of functions at different stages in the cycle. To further characterize its potential roles during cycle progression, one objectives this study was to systematically examine expression all three subunits achieve objective, we examined levels CK2α, CK2α′ CK2β on immunoblots as well activity samples prepared from: (i) elutriated populations MANCA (Burkitt lymphoma) cells, (ii) serum-stimulated GL30-92/R (primary human fibroblasts) cells (iii) drug-arrested chicken bursal lymphoma BK3 A cells. On immunoblots, observed significant co-ordinate increase CK2α following serum stimulation quiescent fibroblasts. By comparison, no major fluctuations were detected any other Furthermore, did not observe dramatic differences between relative However, amount arrested with nocodazole. We also extracts or immunoprecipitates Of particular interest is observation changed nocodazole-arrested Since maximally phosphorylated these result suggests phosphorylation by p34cdc2 does affect catalytic CK2. increased incubation vitro. activation independent ATP speculate may have an associated factor stimulates activity. Collectively, observations mitotic affects vitro suggest regulatory division. (Mol Cell Biochem 191: 213–222, 1999)