Altered Plasticity of Glycogen Phosphorylase in Forebrain Gliosomes Obtained from Insulinoma Patients

摘要: We investigated a control model of hypoglycemia-exposed brain tissues from small series patients with insulinoma, immediately dissect them, and perform differential cold centrifugation to obtain gliosomes examine alterations glycogenolytic mechanisms. The BB as well MM isoforms glycogen phosphorylase enzymatic protein expression remained unaltered between insulinoma subjects within the gliosomes. However, in form that was potentially activated several folds on placing glucose-free medium. This examined by its increased interaction kinase A. Inhibitors used controls. Furthermore, we demonstrated glucose-depleted medium enhanced production both ATP lactate It is possible portion glucose obtained breakdown circuited through glycolytic pathways generate ATP. has been reported earlier plays major role glutamate uptake, thus preventing seizure during active bouts hypoglycemia. Lactate shuttle astrocytes potential mechanism balance neuronal bioenergetics events Newer approaches pharmacologically modulate may prove be rational approach for neuroprotective therapy this common clinical syndrome