G1/S control of anchorage-independent growth in the fibroblast cell cycle.

摘要: We have developed methodology to identify the block anchorage-independent growth and position it within fibroblast cell cycle. Results with NRK fibroblasts show that mitogen stimulation of G0/G1 transition G1-associated increases in size are minimally affected by loss anchorage. In contrast, induction G1/S cycle genes DNA synthesis is markedly inhibited when anchorage blocked. Moreover, we demonstrate anchorage-dependent maps late G1 shortly before activation p34cdc2-like kinase. The was also detectable NIH-3T3 cells. Our results: (a) distinguish control progression factors anchorage; (b) indicate mediates fibroblasts; (c) a physiologic circumstance which phenotype mammalian arrest would closely resemble Saccharomyces cerevisiae START. close correlation between independence vitro tumorigenicity vivo emphasizes key regulatory role for