Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens

作者: Benjamin F. Grewe , Fabian F. Voigt , Marcel van ’t Hoff , Fritjof Helmchen

DOI: 10.1364/BOE.2.002035

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摘要: Functional two-photon Ca^ 2+-imaging is a versatile tool to study the dynamics of neuronal populations in brain slices and living animals. However, population imaging is typically restricted to a single two-dimensional image plane. By introducing an electrically tunable lens into the excitation path of a two-photon microscope we were able to realize fast axial focus shifts within 15 ms. The maximum axial scan range was 0.7 mm employing a 40x NA0. 8 water immersion objective, plenty for typically required ranges of 0.2–0.3 mm. By …

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