Lentiviral Vector–Mediated Gene Delivery into Human Embryonic Stem Cells

摘要: Human embryonic stem cells (hESCs) are pluripotent derived from the inner cell mass of preimplantation embryos. These can be cultured for long periods as undifferentiated and still retain their potential to give rise types representing all three germinal layers. Given unique properties, hESCs expected serve an invaluable tool basic applied research. However, exploit remarkable potentials, development effective strategies genetic modification is required. Lentiviral-based vectors offer attractive system efficient gene delivery into hESCs. lentiviruses, a group complex retroviruses that cause slow chronic immunodeficiency diseases in humans animals. Gene by lentiviruses has following advantages: (1) lentiviral efficiently transduce hESCs; (2) they integrate host-cell genome, thus promoting stable transgene expression; (3) expression not significantly silenced (4) transduced self-renewal potential. In recent years, we others have developed protocols transduction advanced modified replication-defective lentiviral-based vectors. Transduction these resulted high was maintained over cultivation after differentiation. This chapter focuses on methods use