The claudin gene family: expression in normal and neoplastic tissues.

摘要: The claudin (CLDN) genes encode a family of proteins important in tight junction formation and function. Recently, it has become apparent that CLDN gene expression is frequently altered several human cancers. However, the exact patterns various cancers unknown, as only limited number have been investigated few tumors. We identified all from Genbank we used large public SAGE database to ascertain 21 266 normal neoplastic tissues. Using real-time RT-PCR, also surveyed subset 13 24 show claudins represent highly related proteins, with claudin-16, -23 being most different others. From silico analysis RT-PCR data, find appear decreased cancer, while CLDN3, CLDN4, CLDN7 are elevated malignancies such those originating pancreas, bladder, thyroid, fallopian tubes, ovary, stomach, colon, breast, uterus, prostate. Interestingly, CLDN5 expressed vascular endothelial cells, providing possible target for antiangiogenic therapy. CLDN18 might biomarker gastric cancer. Our study confirms previously known identifies new ones, which may applications detection, prognosis therapy In particular identify express CLDN3 CLDN4. These ideal candidates novel developed based on CPE, toxin specifically binds claudin-3 claudin-4.