Isolation of Distinct Progenitor Stem Cell Populations from Dental Pulp

摘要: The present study compared the cellular characteristics of progenitor stem cell populations in adult dental pulp, isolated by different methods utilizing 2 features biology. One population expressing high levels beta1 integrin was preferential selection adherent cells to fibronectin over 20 min. In an alternative approach, embryonic neural crest marker, low-affinity nerve growth factor receptor (LANGFR), were selected magnetic-activated sorting. For each method, clonal lines established and expanded culture. clone derived via respective examined for embryonic/progenitor markers immunocytochemistry RT-PCR. Both demonstrated expression stro-1 stained positive vimentin, demonstrating mesenchymal lineage. Of note, LANGFR additional CD105 Notch 2. both populations, cultures ability differentiate into osteoblasts, adipocytes chondrocytes. These results would suggest potential isolation exhibiting terms their nature. derive from a common origin is discussed.