Downregulated Gene Expression in Human Palate Fibroblasts after Cyclosporin A Treatment

摘要: Background Cyclosporin A is a powerful immunosuppressive drug with considerable impact on transplants and able to modify extracellular matrix (ECM) composition. It has recently been demonstrated that cyclosporin stimulates the production of cytokine family. Cytokines such as interleukin, transforming growth factor β 1 , bone morphogenetic protein induce deposition glycosaminoglycans (GAGs), proteoglycans, collagen fibers in connective ECM. ECM composition very important for normal tissue development function. In this work, we examine effects caused by cultures human palate fibroblasts order evaluate II, II membrane receptor induction GAG changes hyaluronic acid, heparin sulfate, chondroitin sulfate. Methods Palate were maintained 24 h serum-free 199 medium containing 5 μg/mL 3 H glucosamine hydrochloride. After time, TGF BMP receptors determined microarray analysis classes biochemical method. Results The results show TGFβ are significantly inhibited A-treated compared controls, whereas IL-1R2 stimulated. behavior total intra- GAGs increased cultures, ratio between non-sulfated/sulfated decreases ( p ≤0.01) vis-a-vis controls. Conclusions Because they form highly complicated macromolecular network ECM, which provides an indication cell function gene expression modulates activities, related modification functions. Our data causes through alterations cytokines respective linkages.