The patB gene product, required for growth of the cyanobacterium Anabaena sp. strain PCC 7120 under nitrogen-limiting conditions, contains ferredoxin and helix-turn-helix domains.

摘要: A mutant of Anabaena sp. strain PCC 7120, called PAT-2, that grows poorly under nitrogen-fixing conditions, has been isolated. The heterocysts the develop much more slowly than those wild type and are spaced closely in an older culture type. wild-type gene complements mutation patB, was isolated characterized. predicted 529-amino-acid PatB protein contains a region very similar to Fe4S4 bacterial-type ferredoxins near its N terminus helix-turn-helix motif C terminus. This pattern domains resembles transcriptional regulators both prokaryotes eukaryotes. PAT-2 is deletion G at nucleotide 1342 patB gene, resulting loss 62-amino-acid fragment from protein, including motif.