A whole-cell patch clamp technique which minimizes cell dialysis
作者： J.R. Hume , R.N. Leblanc
DOI: 10.1007/BF00231003
关键词:
摘要: A variant of the whole-cell patch clamp technique is described which allows measurement ionic currents in small cells while minimizing cell dialysis with pipette solution. The involves application negative pressure to inside (< 1 µm) tip diameter pipettes placed on surface achieve high resistance seals and membrane rupture. has been used successfully a variety different types study carried by Ca K, generated exchange carriers as well electrical coupling between cells. Overall, seems suited for cells, provides an alternative conventional clamping techniques necessitate intracellular dialysis.
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W. R. Loewenstein, Y. Kanno, S. J. Socolar, Quantum jumps of conductance during formation of membrane channels at cell-cell junction. Nature. ,vol. 274, pp. 133- 136 ,(1978) , 10.1038/274133A0
ERWIN NEHER, BERT SAKMANN, Single-channel currents recorded from membrane of denervated frog muscle fibres Nature. ,vol. 260, pp. 799- 802 ,(1976) , 10.1038/260799A0
H F Brown, A Clark, S J Noble, Analysis of pace-maker and repolarization currents in frog atrial muscle. The Journal of Physiology. ,vol. 258, pp. 547- 577 ,(1976) , 10.1113/JPHYSIOL.1976.SP011435
R. Inoue, K. Kitamura, H. Kuriyama, Two Ca-dependent K-channels classified by the application of tetraethylammonium distribute to smooth muscle membranes of the rabbit portal vein. Pflügers Archiv: European Journal of Physiology. ,vol. 405, pp. 173- 179 ,(1985) , 10.1007/BF00582557
R W Hadley, J R Hume, An intrinsic potential‐dependent inactivation mechanism associated with calcium channels in guinea‐pig myocytes. The Journal of Physiology. ,vol. 389, pp. 205- 222 ,(1987) , 10.1113/JPHYSIOL.1987.SP016654
O. P. Hamill, A. Marty, E. Neher, B. Sakmann, F. J. Sigworth, Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches Pflügers Archiv - European Journal of Physiology. ,vol. 391, pp. 85- 100 ,(1981) , 10.1007/BF00656997
A Uehara, J R Hume, Interactions of organic calcium channel antagonists with calcium channels in single frog atrial cells. The Journal of General Physiology. ,vol. 85, pp. 621- 647 ,(1985) , 10.1085/JGP.85.5.621
J R Hume, A Uehara, Ionic basis of the different action potential configurations of single guinea-pig atrial and ventricular myocytes. The Journal of Physiology. ,vol. 368, pp. 525- 544 ,(1985) , 10.1113/JPHYSIOL.1985.SP015874
K.S. Lee, N. Akaike, A.M. Brown, The suction pipette method for internal perfusion and voltage clamp of small excitable cells. Journal of Neuroscience Methods. ,vol. 2, pp. 51- 78 ,(1980) , 10.1016/0165-0270(80)90045-X
R. Inoue, K. Okabe, K. Kitamura, H. Kuriyama, A newly identified Ca2+ dependent K+ channel in the smooth muscle membrane of single cells dispersed from the rabbit portal vein. Pflügers Archiv: European Journal of Physiology. ,vol. 406, pp. 138- 143 ,(1986) , 10.1007/BF00586674