Soybean GmAOC3 promotes plant resistance to the common cutworm by increasing the expression of genes involved in resistance and volatile substance emission in transgenic tobaccos

摘要: The evaluation and use of endogenous soybean genes is an effective strategy to minimize the yield losses caused by insects. Allene oxide cyclase (AOC) catalyzes most important step in biosynthesis jasmonate (JA), which plays a crucial role plant defense against In this study, GmAOC3 insect resistance was evaluated. Real-time PCR results indicate that uniquely rapidly activated attained peak expression leaves after attack common cutworm (CCW). bioassays, transgenic lines overexpressing were significantly less damaged than wild-type plants, relative growth rate CCW fed with from lower plants. Electron microscopy revealed density leaf trichomes greater tobacco. Several physiological morphological indicators, including JA, phenolic content levels putrescine N-methyltransferase (PMT) proteinase inhibitor (PI) genes, phenylalanine ammonia lyase (PAL) activity volatile substances, increased plants GmAOC3. Our findings can be used as resource for breeding.