Expression, characterization, and purification of a phosphorylated rabies nucleoprotein synthesized in insect cells by baculovirus vectors.
作者： Christophe PrÉhaud , Raymond D Harris , Vilmos Fulop , Chong-Lek Koh , Joseph Wong
DOI: 10.1016/0042-6822(90)90346-S
关键词:
摘要: A baculovirus expression vector (AcNPV3) derived from the nuclear polyhedrosis virus of Autographa californica (AcNPV) was prepared containing complete coding region nucleoprotein (N) gene rabies (Gif-sur-Yvette clone CVS strain). The placed under control AcNPV polyhedrin promoter and expressed to high levels (66 mg N protein/liter 2 x 10(9) cells) by recombinant using a Spodoptera frugiperda cell line. Using available antisera, it established that antigenic characteristics protein were similar comparison with those native virus. Characterization that, like mammalian cell-grown virus, phosphorylated. induced antibodies in mice reacted strongly viral protein. recovered insect cells differential centrifugation followed ion exchange chromatography. represents source authentic suitable for diagnosis as well structural studies.
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