Functional Genomic and Serological Analysis of the Protective Immune Response Resulting from Vaccination of Macaques with an NS1-Truncated Influenza Virus.

摘要: We are still inadequately prepared for an influenza pandemic due to the lack of a vaccine effective subtypes which majority human population has no prior immunity and could be produced rapidly in sufficient quantities. There is therefore urgent need investigate novel vaccination approaches. Using combination genomic traditional tools, this study compares protective efficacy macaques intrarespiratory live virus by truncating NS1 A/Texas/36/91 (H1N1) with that conventional based on formalin-killed whole virus. After homologous challenge, animals live-vaccine group had greatly reduced viral replication pathology lungs upper respiratory inflammation. They also lesser induction innate immune pathways interferon-sensitive genes bronchial epithelium. This postchallenge response contrasted shortly after vaccination, when more expression was observed cells from group. suggested strong NS1-truncated virus, followed greater maturity response, as demonstrated robust virus-specific CD4+ T-cell proliferation, immunoglobulin G production, transcriptional T- B-cell lung tissue. In conclusion, single tract inoculation protecting nonhuman primates challenge. protection achieved absence significant or long-lasting adverse effects through adaptive response.