Differential expression profiling of orange-spotted grouper larvae, Epinephelus coioides (Hamilton), that survived a betanodavirus outbreak.

摘要: Nervous necrosis virus (NNV), a piscine nodavirus, has caused serious viral nervous and encephalopathy retinopathy in hatchery-reared larvae juveniles of wide range marine teleost species worldwide the last two decades. Although mortality NNV-infected is nearly 100%, there are still some that survive this catastrophe. To comprehensively understand variations these survivors at molecular level, we collected orange-spotted grouper survived an NNV outbreak indoor hatchery southern Taiwan to study differential gene expression. Healthy with high, medium low levels detected were compared morbid using 9600-clone-containing larva cDNA microarray, expression was further confirmed by quantitative real-time polymerase chain reaction. Significant variation exists healthy larvae. The following genes upregulated: adenylate kinase 1-2, myosin binding protein H-like, light 2, 3, tropomyosin, fast/white muscle troponin T embryonic isoform, parvalbumin 1 2 genes. downregulated: apolipoprotein A-I, trypsinogen, pyruvate astacin-like metalloprotease. Moreover, immunoglobulin M heavy transcription significantly higher had high levels, indicating humoral immunity might protect organisms from infection. These results suggest non-immune-related may have played important roles survival during larval metamorphosis stage, after betanodavirus