Application of the “two-slit” photometric technique to the measurement of microvascular volumetric flow rates

作者: Herbert H. Lipowsky , Benjamin W. Zweifach

DOI: 10.1016/0026-2862(78)90009-2

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摘要: Abstract In vitro studies of red cell suspensions flowing through glass tubes were performed to provide additional details on the empirical relationship between velocity measured by “twoslit” photometric technique along vessel centerline, V , and mean cells plus plasma, Vmean. Small bore (17- 60-μm internal diameter) used simulate a blanket application this method microcirculation. The previously established ratio, = 1.6 was found be valid within 5 10% in these for velocities physiological range. For tube diameters decreasing from 60 17 μm, increase slightly but still ratio. Analysis earlier vivo single-file motion 6- 10-μm capillaries, suggests that below 10 should approach value order 1.3, or 19% factor.

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