Preparation of protein extracts from yeast.
作者： Barbara Dunn , C. Richard Wobbe
DOI: 10.1002/0471142727.MB1313S23
关键词:
摘要: Three protocols are presented for preparing protein extracts; they differ primarily in the way cells broken. In basic protocol, enzymatically converted to spheroplasts, which then lysed by a combination of osmotic shock and Dounce homogenization. A support protocol isolating intact nuclei differential centrifugation is also presented. An alternate describes mechanical breakage vortexing presence glass beads. second growing frozen immediately liquid nitrogen grinding an industrial-strength blender nitrogen.
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