Glutamine synthetase and glutamate dehydrogenase contribute differentially to proline accumulation in leaves of wheat (Triticum aestivum) seedlings exposed to different salinity.
作者： Zhi-Qiang Wang , Yong-Ze Yuan , Ji-Quan Ou , Qing-Hua Lin , Chu-Fu Zhang
DOI: 10.1016/J.JPLPH.2006.05.001
关键词:
摘要: Summary To investigate the roles of ammonium-assimilating enzymes in proline synthesis under salinity stress, activities glutamine synthetase (GS; EC 6.3.1.2) and NADH-dependent glutamate dehydrogenase (NADH-GDH; 1.4.1.2) were determined leaves wheat (Triticum aestivum) seedlings exposed to salt stress at 150 300 mM NaCl for 5 d. At lower salinity, only GS activity increased markedly. NaCl, however, NADH-GDH while decreased. A significant accumulation was found high-salinity exposure glutamate, a precursor, dramatically both low high salinity. These data suggests that GS-catalysis might be main pathway seems preferentially produced through process catalyzed by NADH-GDH. The increase ammonium salinity-stressed have resulted from photorespiration, which is responsible higher activity. Δ1-pyrroline-5-carboxylate reductase (P5CR; 1.5.1.2) significantly enhanced but remained unchanged 150 mM. Δ1-Pyrroline-5-carboxylate (P5CS) did not show specific response, indicating P5CR limiting step
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