A novel 57 kDa peroxisomal membrane polypeptide detected by monoclonal antibody (PXM1a/207B).
作者： Tsuneo Imanaka , Paul B. Lazarow , Tatsuya Takano
DOI: 10.1016/0005-2736(91)90401-S
关键词:
摘要: BALB/c mice were immunized with peroxisomal membranes prepared from rat liver. Spleen cells fused myeloma (P3/U1) and the hybridomas selected using membranes. A monoclonal antibody (PXM1a/207B) which recognized was selected. Using antibody, a novel 57 kDa polypeptide identified in membrane fraction. Immunoblot analysis of subcellular fractions demonstrated that present exclusively The partially digested by trypsin chymotrypsin under conditions where particles remained intact, indicating is exposed to cytosolic face membrane. amount increased parallel proliferation peroxisomes administration clofibrate.
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