A Potential New Human Pathogen Belonging to Helicobacter Genus, Identified in a Bloodstream Infection

摘要: We isolated from aerobic and anaerobic blood culture bottles a febrile patient, Helicobacter-like Gram negative, rod-shaped bacterium that MALDI-TOF MS failed to identify. Blood agar cultures incubated in microaerobic atmosphere revealed motile negative rod, which was oxidase, catalase, nitrate reductase, esterase, alkaline phosphatase positive. It grew at 42°C with no detectable urease activity. Antimicrobial susceptibility testing showed the organism susceptible beta-lactams, gentamicin, erythromycin, tetracycline but resistant ciprofloxacin. Electronic microscopy analysis 3 × 0.5 μm curved rod harboring two sheathed amphitrichous flagella. Whole genome sequencing 1,708,265 base-pairs long GC content of 37.80% total 1,697 coding sequences. The genomic analyses using nucleotide sequences 16S rRNA gene, hsp60 gyrB genes, as well GyrA protein sequence, results Average Nucleotide Identity silico DNA-DNA hybridization suggest evidence for novel Helicobacter species close equorum belonging group enterohepatic species. As soon particular peptide mass fingerprint this pathogen is added spectral databases, technology will improve its identification clinical specimens, especially case "sterile infection". propose associate present strain Latin name place isolation; Caesarodunum (Tours, France) "Helicobacter caesarodunensis" further description new bacterium.