Determining SNP allele frequencies in DNA pools.

摘要: Single nucleotide polymorphisms (SNPs) are among the most common types of polymorphism used for genetic association studies. A method to allow accurate quantitation their allele frequencies from DNA pools would both increase throughput and decrease costs large-scale genotyping. However, date, pooling studies have concentrated on use microsatellite polymorphisms. In case SNPs that restriction fragment length (RFLPs), tended methods frequency rely densitometric evaluation bands an autoradiograph. Radiation-based well-known drawbacks, we present two alternative determination SNP frequencies. For RFLPs, agarose gel electrophoresis digested PCR products with ethidium bromide staining combined analysis images a PC. all SNP, allele-specific fluorescent probes in Taqman assay determine relative different alleles. Both gave reproducible results, suggesting they suitable experiments.