Expression and Transcriptional Regulation of the PD-Iα/Autotaxin Gene in Neuroblastoma

摘要: Autotaxin (ATX) is a newly found autocrine tumor cell motility-stimulating factor. ATX member of the ecto-phosphodiesterase I (PD-I)/nucleotide pyrophosphatase family. PD-Iα was as brain-type I/nucleotide pyrophosphatase. and are alternative splicing products from one gene. stimulates motility A2058 melanoma cells in vitro ; however, it has not been known if PD-Iα/ATX expressed naturally occurred human tumors. In this study, we examined expression gene neuroblastoma tissues stimulating activity recombinant on investigated its transcriptional regulatory mechanism line. The primary patients to varying degrees. This also SMS-KAN We identified both isoforms, ATX, these cells. stimulated at low nanomolar concentration. situated promoter region, which essential for transcription cells, -287 -254 nucleotides by assay. gel-shift assay revealed that there exists nuclear protein binds region. These new insights about will help us understand metastatic neuroblastoma.