Factors influencing drug protein binding in patients with end stage renal failure.

摘要: Investigations were undertaken to evaluate which uraemic solutes decrease drug protein binding. This was done by performing HPLC-fractionation of biological fluids and studying the effect addition a lyophilisate each fraction normal plasma containing standard quantities radiolabelled drugs. From first study, based only on fractionation ultrafiltrate with an HPLC-gradient mainly aimed at elution hydrophilic compounds, hippuric acid appeared be major binding inhibitor for theophylline phenytoin. The problem this approach that it did not include compounds most substantial Therefore, studies planned fractionate deproteinized sera, but necessary define deproteinisation methods gave highest yields bound ligand. Heat denaturation found one effective methods. When serum, deproteinised method, added plasma, higher capacity displace from sites compared identical volume same samples. Fractionation sample HPLC revealed larger number fractions able inhibit