Fertilization of partially jellied and jellyless oocytes of the frog Rana pipiens.

摘要: The oocyte of Rana pipiens is coated by three visible jelly layers designated V1, V2, and V3 from inner to outer. Oocytes with V1 were rarely fertilized, while oocytes V3, fertilized. lost their fertilizability more rapidly after being placed in 10% Ringer's than did V3. When jellied dejellied KCN, they not fertilized on insemination. addition a water extract (egg-water) insured fertilization oocytes. finding that the factors necessary for are diffusible decreases importance locating them given layer. activity egg-water survived lyophilization was heatlabile. Both dialyzable non-dialyzable fraction required insure high frequency fertilization. Egg-water had be present sperm order occur. Sperm incubated fertilizing ability time. Mature body cavity oocytes, which been oviduct, at low under same conditions resulted Similarly, toad Bufo americanus or frog egg-water,