Fast solubilization of recalcitrant cellulosic biomass by the basidiomycete fungus Laetisaria arvalis involves successive secretion of oxidative and hydrolytic enzymes

摘要: Background: Enzymatic breakdown of lignocellulosic biomass is a known bottleneck for the production high-value molecules and biofuels from renewable sources. Filamentous fungi are predominant natural source enzymes acting on lignocellulose. We describe extraordinary cellulose-deconstructing capacity basidiomycete Laetisaria arvalis, soil-inhabiting fungus. Results: The L. arvalis strain displayed to grow wheat straw as sole carbon fully digest cellulose filter paper. cellulolytic activity exhibited in secretomes was up 7.5 times higher than that reference Trichoderma reesei industrial strain, resulting significant improvement glucose release steam-exploded straw. Global transcriptome secretome analyses revealed produces unique repertoire carbohydrate-active fungal taxa, including complete set cellulose. Temporal indicated unusual degradation efficiency relies its early response source, finely tuned sequential secretion several lytic polysaccharide monooxygenases hydrolytic targeting Conclusions: present study illustrates adaptation litter-rot fungus rapid recalcitrant plant biomass. capabilities this result rapid, selective successive oxidative enzymes. These expressed at critical during may inspire design improved enzyme cocktails conversion cell wall resources into fermentable sugars.