4 – Molecular Analysis of Genetic and Epigenetic Alterations in Human Cells

摘要: Publisher Summary Recombinant DNA technology and sequencing techniques allow mutations induced in mammalian cells to be analyzed at the molecular level. If gene which are has been cloned, gross structural alterations readily detected by Southern analysis. Point or small deletions much more difficult analyze. Several different mutation systems have subjected Mutations endogenous genes analysed cloning of entire mutated from genome. Endogenous ideal system use. viral recovery particles their DNA. Shuttle vectors designed a target is inserted into plasmid that capable replicating both bacterial cells. The inside cell, after it recovered cell used transform suitable indicator bacteria easily identified bacteria.