A pcr-based multiplex assay for determining haplotype

摘要: The invention provides a method for determining haplotype in template DNA sequence having first and second polymorphic marker comprising combining single tube sequence, forward primers that are allele-specific the marker, reverse Stoffel fragment polymerase; conducting polymerase chain reaction (PCR) amplifications to produce an amplification product; analyzing product identify which pair of said generated wherein is determined by identification primer pair.