Ibuprofen-loaded poly(trimethylene carbonate-co-ε-caprolactone) electrospun fibres for nerve regeneration

摘要: The development of scaffolds that combine the delivery drugs with physical support provided by electrospun fibres holds great potential in field nerve regeneration. Here it is proposed incorporation ibuprofen, a well-known non-steroidal anti-inflammatory drug, statistical copolymer poly(trimethylene carbonate-co-e-caprolactone) [P(TMC-CL)] to serve as drug system enhance axonal regeneration context spinal cord lesion, limiting inflammatory response. P(TMC-CL) were from mixtures dichloromethane (DCM) and dimethylformamide (DMF). solvent mixture applied influenced fibre morphology, well mean diameter, which decreased DMF content solution increased. Ibuprofen-loaded prepared solutions containing 5% ibuprofen (w/w polymer). Increasing 10% led jet instability, resulting formation less homogeneous fibrous mesh. Under optimized conditions, drug-loading efficiency was above 80%. Confocal Raman mapping showed no preferential distribution fibres. physiological conditions released 24 h. release process being diffusion-dependent for DCM solutions, contrast DCM-DMF where burst occurred. biological activity demonstrated using human-derived macrophages. prostaglandin E2 cell culture medium reduced when cells incubated ibuprofen-loaded fibres, confirming significance strategy presented. Overall, this study constitutes an important contribution design P(TMC-CL)-based conduit properties.