Analysis of a laminar-flow diffusional mixer for directed self-assembly of liposomes.

摘要: The present work describes the operation and simulation of a microfluidic laminar-flow mixer. Diffusive mixing takes place between core solution containing lipids in ethanol sheath aqueous buffer, leading to self assembly liposomes. Present device architecture hydrodynamically focuses lipid into cylindrical positioned at center channel 125 × 125-μm2 cross-section. Use produces liposomes size range 100–300 nm, with larger forming greater ionic strength lower concentration solution. Finite element simulations compute distributions solutes axial distances than 100 widths. These reduce computation time enable long by utilizing hexahedral elements flow region fine tetrahedral hydrodynamic focusing region. meshing technique is generally useful for channels fully implementable using comsol Multiphysics. Confocal microscopy provides experimental validation fluorescent solutions fluorescein or enhanced green protein.