Detection of Balamuthia mandrillaris DNA in the storage case of contact lenses in Germany.

摘要: Acanthamoeba spp. are frequently the etiological agents of a severe form sight-threatening keratitis, called keratitis. The contact lens storage solution patient with keratitis unknown genesis was screened using our diagnostic tools to detect potentially pathogenic free-living amoebae (FLA). Culture methods and triplex quantitative real-time polymerase chain reaction (qPCR) targeting spp., Naegleria fowleri, Balamuthia mandrillaris were used in context this routine screening. While no detected by culture, qPCR specifically DNA B. mandrillaris. This FLA is known as agent fatal encephalitis humans other mammals, amoebic (BAE). A fragment 18S rDNA gene amplified from sample showed 99 % sequence identity sequences GenBank. To best knowledge, first report found association lenses. Although viable amoeba obtained culturing efforts, verification demonstrates how easily pathogen might come into close humans.