Development of murine bariatric surgery models: lessons learned.

摘要: Abstract Roux-en-Y gastric bypass (RYGB) improves comorbidities such as diabetes and hypertension lowers the risk of obesity-related cancers. To better understand physiologic genetic influences bariatric surgery, a reliable murine model is needed that can be extended to genetically engineered mice. Given complexity these procedures, few researchers have successfully implemented techniques beyond larger rodent models. The purpose our study was develop technically feasible reproducible for RYGB sleeve gastrectomy (SG). Mice were converted liquid diet perioperatively without fasting housed in groups on raised wire platforms. SG involved significant reduction stomach volume followed by multilayer repair gastrotomy. procedure consisted side-to-side, functional end-to-side bowel anastomoses exclusion medial gastroesophageal junction. Sham surgeries enterotomies gastrotomy primary resection or rerouting. Survival after incorporation aforementioned 100% group 41% at 1 mo surgery. Only 26% mortality attributed leak, obstruction, stricture; majority postoperative due stress, dumping, malnutrition. Much survival challenge this surgical related perioperative husbandry, which expected given their small stature poor response stress. Utilization described will increase feasibility challenging allowing understanding mechanisms explain beneficial effects