Cloning of the GATA-binding protein that regulates endothelin-1 gene expression in endothelial cells.

摘要: Previously, we have identified two regions (A and B) of the endothelin-1 promoter that are important for expression this gene in cultured vascular endothelial cells. The cis-acting sequence one these (Region A) includes core binding motif GATA, raising possibility region DNA mediates a member GATA-binding protein family. In report, describe use polymerase chain reaction conjunction with cDNA cloning to characterize expressed nucleotide cell clones is highly homologous chicken GATA-2 (NF-E1b) gene, indicating our encode human transcript. By RNA blot analysis, lines derived from number different tissues. Transactivation experiments utilizing eukaryotic vectors indicate interacts GATA increase transcription reporter genes both BAEC HeLa These data provide first evidence non-erythroid target regulated by may more broad role transcriptional regulation than erythroid-specific GATA-1 protein.