Rabbit corneal epithelial cells adhere to two distinct heparin-binding synthetic peptides derived from fibronectin
作者： D. L. Mooradian , L. T. Furcht , A. P. N. Skubitz , J. B. Mccarthy , J. D. Cameron
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摘要: Fibronectin plays an important role in corneal reepithelialization during wound healing. In this study, rabbit epithelial (RCE) cell adhesion to fibronectin was further defined using proteolytic fragments of and chemically synthesized peptides derived from the amino acid sequence fibronectin. RCE cells adhere intact fibronectin, 75 kD fragment containing RGDS (Arg-GlyAsp-Ser) adhesion-promoting sequence, 33/66 promoting/heparin-binding The partially inhibited suggesting that these represent distinct sites used by Two FN-C/H-I (YEKPGSPPREWPRPRPGV) FN-C/H-III (YRVRVTPKEKTGPMKE), directly promoted cells. As evidence play a we have shown soluble on surfaces coated with fragments. addition, polyclonal IgG against FN-C/ H-I blocked fragments, confirming sequences within contrast, two previously described which variety types, FN-C/H-II (KNNQKSEPLIGRKKT) CS-1 (DELPQLVTLPHPNLHGPEILDVPST), did not support adhesion. These data suggest has complex molecular basis is mediated multiple domains, at least are located car boxy 1-terminal heparin-binding/cell domains Invest Ophthalmol Vis Sci 33:3034-3040,1992
arvojournals.org参考文章(35)
T Otori, K Watanabe, S Nakagawa, K M Yamada, M B Berman, T Nishida, A peptide from fibronectin cell-binding domain inhibits attachment of epithelial cells. Investigative Ophthalmology & Visual Science. ,vol. 29, pp. 1820- 1825 ,(1988)
K. Sekiguchi, M. Fukuda, S. Hakomori, Domain structure of hamster plasma fibronectin. Isolation and characterization of four functionally distinct domains and their unequal distribution between two subunit polypeptides. Journal of Biological Chemistry. ,vol. 256, pp. 6452- 6462 ,(1981) , 10.1016/S0021-9258(19)69186-6
M Berman, D Aiken, E Manseau, M Law, Ulceration is correlated with degradation of fibrin and fibronectin at the corneal surface. Investigative Ophthalmology & Visual Science. ,vol. 24, pp. 1358- 1366 ,(1983)
Anne Woods, John R Couchman, M Höök, None, Heparan sulfate proteoglycans of rat embryo fibroblasts. A hydrophobic form may link cytoskeleton and matrix components. Journal of Biological Chemistry. ,vol. 260, pp. 10872- 10879 ,(1985) , 10.1016/S0021-9258(19)85163-3
G G Koliakos, K Kouzi-Koliakos, L T Furcht, L A Reger, E C Tsilibary, The binding of heparin to type IV collagen: domain specificity with identification of peptide sequences from the α1(IV) and α2(IV) which preferentially bind heparin Journal of Biological Chemistry. ,vol. 264, pp. 2313- 2323 ,(1989) , 10.1016/S0021-9258(18)94178-5
A C Rapraeger, M Bernfield, Heparan sulfate proteoglycans from mouse mammary epithelial cells. A putative membrane proteoglycan associates quantitatively with lipid vesicles. Journal of Biological Chemistry. ,vol. 258, pp. 3632- 3636 ,(1983) , 10.1016/S0021-9258(18)32712-1
M J Humphries, A Komoriya, S K Akiyama, K Olden, K M Yamada, Identification of two distinct regions of the type III connecting segment of human plasma fibronectin that promote cell type-specific adhesion. Journal of Biological Chemistry. ,vol. 262, pp. 6886- 6892 ,(1987) , 10.1016/S0021-9258(18)48327-5
LLOYD A. CULP, GABRIELE MUGNAI, KRISTINE LEWANDOWSKA, ELIZABETH A. VALLEN, MARY A KOSIR, KATHRYN L. HOUMIEL, Heparan Sulfate Proteoglycans of Ras‐Transformed 3T3 or Neuroblastoma Cells Annals of the New York Academy of Sciences. ,vol. 556, pp. 194- 216 ,(1989) , 10.1111/J.1749-6632.1989.TB22504.X
A P Mould, L A Wheldon, A Komoriya, E A Wayner, K M Yamada, M J Humphries, Affinity chromatographic isolation of the melanoma adhesion receptor for the IIICS region of fibronectin and its identification as the integrin alpha 4 beta 1. Journal of Biological Chemistry. ,vol. 265, pp. 4020- 4024 ,(1990) , 10.1016/S0021-9258(19)39696-6
Tuyet-Mai M. Phan, C. Stephen Foster, S. Arthur Boruchoff, Luba M. Zagachin, Robert B. Colvin, Topical fibronectin in the treatment of persistent corneal epithelial defects and trophic ulcers American Journal of Ophthalmology. ,vol. 104, pp. 494- 501 ,(1987) , 10.1016/S0002-9394(14)74107-8