Suppression of KV7/KCNQ potassium channel enhances neuronal differentiation of PC12 cells.

摘要: Membrane potential shift driven by electrical activity is critical in determining the cell fate of proliferation or differentiation. As such, ion channels that underlie membrane play an important role proliferation/differentiation. KV7/KCNQ potassium are resting potentials many neuronal cells. However, these differentiation not well studied. In present study, we used PC12 cells as primary cultured rat cortical neurons to study and mechanism NGF induced into neuron-like with growth neurites showing typical cone-like extensions. The Kv7/KCNQ blocker XE991 promoted NGF-induced neurite outgrowth, whereas opener retigabine (RTG) inhibited outgrowth. M-type Kv7 likely involved regulating because overexpression KCNQ2/Q3 suppression shRNA growth. depolarization possibly underpins enhanced Kv7/KCNQ. Additionally, high extracellular K(+) also Finally, T-type Ca(2+) may be membrane-depolarization-induced This provides a new perspective for understanding channel function.