Calcineurin as a neuronal marker of human brain tumors

摘要: Calcineurin is one of the calmodulin binding proteins and a Ca2+-dependent calmodulin-stimulated phosphoprotein phosphatase. We used antisera to calcineurin as cell-type-specific marker in order identify neuronal cells rat brain human neoplasms. In normal slices, basal ganglia were stained macroscopically, other areas such cerebral cortex, corpus callosum, cerebellar granular layer pyramidal tract spinal cord lightly identified well. Under light microscope, it was found that only stained, astrocytes, oligodendrocytes, ependymal vessels not. Intracellular distribution staining showed various patterns intensity varying degree. Using PAP method, localization formalin-fixed, paraffin-embedded tissues studied 65 intracranial neoplasms, 11 extracranial The elements neuroblastoma, ganglioglioma, ganglioneuroma retinoblastoma clearly stained. contrast, glioblastoma, astrocytoma, oligodendroglioma, ependymoma, meningioma, neurinoma, pituitary adenoma, craniopharyngioma, hemangioblastoma, hamartoma, lymphoma mesenchymal tumor all negative. Two cases out 5 medulloblastomas but others Although positive tumors disclosed intensities, these results indicated could be new tumors.