Alta frecuencia de mutaciones puntuales en pfcrt de Plasmodium falciparum y emergencia de nuevos haplotipos mutantes en Colombia

摘要: Introduction. Studies on the molecular epidemiology of antimalarial resistance constitute a useful tool to understand events underlying treatment failure and in falciparum malaria Colombia. Several authors have reported efficacy some markers predict drug Plasmodium falciparum. The P. pfcrt gene has been widely characterized this context. Objective. frequency mutations were associated with antimalarials chloroquine, mefloquine, amodiaquine sulfadoxine/ pyrimethamine. Materials methods. A representative sample 172 patients non-complicated was selected from two highly malaria-endemic areas northeastern Colombia, Turbo Bajo Cauca regions. These assessed for response together status codons 72, 74, 75 76 using PCRRFLP approach. Results. high chloroquine (82%) (29%) confirmed, whereas mefloquine combined therapy remained effective. presence T76 mutation confirmed all samples. most common haplotype CMNT (67%). Conclusions. No significant association between specific haplotypes any groups. Two haplotypes, SMET SMNT, first time Twelve percent samples carried both mixed mutant wild-type alleles.