Fast sex identification in wild mammals using PCR amplification of the Sry gene

摘要: Several primer sets were described for PCR amplification of the Sry gene that became very suitable method sex identification in mammals. We tested applicability two one bat and12 rodent species from Central Europe. Rapid DNA extraction with non-ionic detergents, which is large-scale population studies, was used. Primers SRY-A P o m p et al. (1995) did not amplify fragment males Myotis myotis, four Microtus, and Clethrionomys glareolus, while successful Apodemus, Mus, Sciurus vulgaris. On other hand, primers S a n c h e z (1996) gave clear band all species, only exception, i.e. male