Binding of Salmonella strains to immobilized intestinal mucosal preparations from broiler chickens.

摘要: The binding kinetics of radiolabeled Salmonella california 1989/O (mannose-sensitive hemagglutinin-positive [MSHA+]) to immobilized mucus or enterocytes isolated from broiler ceca and inhibition by D-mannose sodium metaperiodate were characteristic adherence mannose-sensitive type 1 fimbriae bacteria eukaryotic mannose-containing receptors. Binding strains (in the presence D-mannose) S. typhimurium S 7471 N (MSHA-, non-fimbriated) indicated non-specific that was characterized less lack carbohydrates prior treatment with metaperiodate. Inhibition pretreatment various enzymes tetramethylurea p-nitrophenol (known disrupt hydrophobic interactions) indicate involvement multiple sites bonding. Strain-specific outer-membrane preparations inhibited a greater extent than did lipopolysaccharide, Escherichia coli preparations, bovine serum albumin.