Compared interest between hair analysis and urinalysis in doping controls: Results for amphetamines, corticosteroids and anabolic steroids in racing cyclists

摘要: In France during a famous bicycle race, the newspapers documented degree in which doping seemed to be supervised some teams by managers and doctors. Use of anabolic steroids other substances was officially banned mid-seventies sports authorities. This policy has been enforced through urine testing before competition. It is well known, however, that latency period all necessary defeat these tests. Nevertheless, hair analysis could promising tool when for periods are not accessible urinalysis any more. We have developed different sensitive methods amphetamines, their esters corticosteroids. For 50 mg were digested with 1 M NaOH, extracted ethyl acetate, derivatized TFA analyzed gas chromatography positive chemical-ionization mass spectrometry. corticosteroids, powdered treated methanol an ultrasonic bath subsequently purified using C18 solid phase extraction column. Analysis realized high performance liquid coupled electrospray-ionization tandem esters, 100 then alkaline NaOH optimum recovery drugs. The two preparations pooled, finally highly twin on aminopropyl silica cartridges. Residue MSTFA prior injection. conducted triple quadrupole spectrometer. Thirty cyclists sampled tested both urine. Amphetamine detected 10 times (out 19 analyses) compared 6 30 analyses). Corticosteroids 5 (methylprednisolone case, triamcinolone acetonide 3 cases hydrocortisone acetate case) 12 (triamcinolone betamethasone 2 cases) Anabolic twice (nandrolone testosterone undecanoate 25 none