TGF-β1-induced miR-202 mediates drug resistance by inhibiting apoptosis in human osteosarcoma.
作者： Zaijun Lin , Dianwen Song , Haifeng Wei , Xinghai Yang , Tielong Liu
DOI: 10.1007/S00432-015-2028-9
关键词:
摘要: A number of studies have demonstrated that microRNAs play a critical role in osteosarcoma progression, therapy and drug resistance. MicroRNA-202 has proven to be dysregulated many human cancer studies. This study aimed explore miR-202 contributions resistance osteosarcoma. expression was measured by real-time PCR patient tissues, cell lines or cells treated with TGF-β1, using siRNA knock down the Smad2, Smad3 Smad4. mimics, inhibitor scramble were transfected into observe effects on apoptosis Moreover, relationships level PDCD4 investigated luciferase reporter assay, Western blotting. We found is overexpressed tissues when compared normal osteoblasts TGF-β1 can induce miR-202. Transfection mimics significantly promotes chemotherapy targeting PDCD4, tumor suppressor which involved apoptosis. In contrast, transfection enhances sensitivity provides new insights miRNA-202 as potential molecular target for chemotherapy.
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sci-hub.se 参考文章(22)
David S. Geller, Richard Gorlick, Osteosarcoma: a review of diagnosis, management, and treatment strategies. Clinical advances in hematology & oncology. ,vol. 8, pp. 705- 718 ,(2010)
Anja Luetke, Paul A. Meyers, Ian Lewis, Heribert Juergens, Osteosarcoma treatment - where do we stand? A state of the art review. Cancer Treatment Reviews. ,vol. 40, pp. 523- 532 ,(2014) , 10.1016/J.CTRV.2013.11.006
Alessandro Franchi, Luisa Arganini, Gianna Baroni, Anna Calzolari, Rodolfo Capanna, Domenico Campanacci, Patrizio Caldora, Laura Masi, Maria Luisa Brandi, Giancarlo Zampi, Expression of transforming growth factor β isoforms in osteosarcoma variants: association of tgfβ1 with high‐grade osteosarcomas The Journal of Pathology. ,vol. 185, pp. 284- 289 ,(1998) , 10.1002/(SICI)1096-9896(199807)185:3<284::AID-PATH94>3.0.CO;2-Z
D Zhang, Z Shi, M Li, J Mi, None, Hypoxia-induced miR-424 decreases tumor sensitivity to chemotherapy by inhibiting apoptosis. Cell Death and Disease. ,vol. 5, ,(2014) , 10.1038/CDDIS.2014.240
Xiang Chen, Armita Bahrami, Alberto Pappo, John Easton, James Dalton, Erin Hedlund, David Ellison, Sheila Shurtleff, Gang Wu, Lei Wei, Matthew Parker, Michael Rusch, Panduka Nagahawatte, Jianrong Wu, Shenghua Mao, Kristy Boggs, Heather Mulder, Donald Yergeau, Charles Lu, Li Ding, Michael Edmonson, Chunxu Qu, Jianmin Wang, Yongjin Li, Fariba Navid, Najat C Daw, Elaine R Mardis, Richard K Wilson, James R Downing, Jinghui Zhang, Michael A Dyer, None, Recurrent Somatic Structural Variations Contribute to Tumorigenesis in Pediatric Osteosarcoma Cell Reports. ,vol. 7, pp. 104- 112 ,(2014) , 10.1016/J.CELREP.2014.03.003
YU-GUANG LI, JIN-HUA HE, LIU YU, ZE-PING HANG, WANG LI, WEN-HONG SHUN, GUO XIAN HUANG, microRNA‑202 suppresses MYCN expression under the control of E2F1 in the neuroblastoma cell line LAN‑5 Molecular Medicine Reports. ,vol. 9, pp. 541- 546 ,(2014) , 10.3892/MMR.2013.1845
Cecil Han, Yunhua Liu, Guohui Wan, Hyun Jin Choi, Luqing Zhao, Cristina Ivan, Xiaoming He, Anil K. Sood, Xinna Zhang, Xiongbin Lu, The RNA-binding protein DDX1 promotes primary microRNA maturation and inhibits ovarian tumor progression. Cell Reports. ,vol. 8, pp. 1447- 1460 ,(2014) , 10.1016/J.CELREP.2014.07.058
Yuan Chen, Shao-Xin Wang, Rui Mu, Xue Luo, Zhao-Shan Liu, Bing Liang, Hai-Long Zhuo, Xiao-Peng Hao, Qiong Wang, Di-Feng Fang, Zhao-Fang Bai, Qian-Yi Wang, He-Mei Wang, Bao-Feng Jin, Wei-Li Gong, Tao Zhou, Xue-Min Zhang, Qing Xia, Tao Li, Dysregulation of the miR-324-5p-CUEDC2 axis leads to macrophage dysfunction and is associated with colon cancer. Cell Reports. ,vol. 7, pp. 1982- 1993 ,(2014) , 10.1016/J.CELREP.2014.05.007
Yi-Kun Zhang, Hua Wang, Yun Leng, Ze-Liang Li, Yue-Feng Yang, Feng-Jun Xiao, Qing-Fang Li, Xie-Qun Chen, Li-Sheng Wang, Overexpression of microRNA-29b induces apoptosis of multiple myeloma cells through down regulating Mcl-1 Biochemical and Biophysical Research Communications. ,vol. 414, pp. 233- 239 ,(2011) , 10.1016/J.BBRC.2011.09.063
Yi Zhang, Dayong Zheng, Yan Xiong, Chengbiao Xue, Gen Chen, Bibo Yan, Qifa Ye, None, miR‐202 suppresses cell proliferation in human hepatocellular carcinoma by downregulating LRP6 post‐transcriptionally FEBS Letters. ,vol. 588, pp. 1913- 1920 ,(2014) , 10.1016/J.FEBSLET.2014.03.030