Optimization of lycopene extraction from tomato cell suspension culture by response surface methodology.
作者： Chi-Hua Lu , Nancy J. Engelmann , Mary Ann Lila , John W. Erdman
DOI: 10.1021/JF801029K
关键词:
摘要: Radioisotope-labeled lycopene is an important tool for biomedical research but currently not commercially available. A tomato cell suspension culture system the production of radioisotope-labeled was previously developed in our laboratory. In current study, goal to optimize extraction efficiency from cultures preparatory high-performance liquid chromatography (HPLC) separation. We employed response surface methodology (RSM), which combines fractional factorial design and a second-degree polynomial model. Tomato cells were homogenized with ethanol, saponified by KOH, extracted hexane, content analyzed HPLC-PDA. varied five factors at levels: ethanol volume (1.33-4 mL/g); homogenization period (0-40 s/g); saturated KOH solution (0-0.67 hexane (1.67-3 vortex (5-25 s/g). Ridge analysis SAS suggested that optimal procedure extract 1 g 1.56 mL 28 s homogenization, 0.29 2.49 17.5 vortex. These conditions predicted RSM confirmed enhance yield standardized more than 3-fold.
acs.org
sci-hub.se 参考文章(14)
R. Mead, D. J. Pike, A Biometrics Invited Paper. A Review of Response Surface Methodology from a Biometric Viewpoint Biometrics. ,vol. 31, pp. 803- 851 ,(1975) , 10.2307/2529809
T. W. Goodwin, The biochemistry of the carotenoids ,(1980)
Kirstie Canene-Adams, Jessica K. Campbell, Susan Zaripheh, Elizabeth H. Jeffery, John W. Erdman, The Tomato As a Functional Food Journal of Nutrition. ,vol. 135, pp. 1226- 1230 ,(2005) , 10.1093/JN/135.5.1226
Charles H. Hennekens, Meir J. Stampfer, Walter Willett, Edward Giovannucci, Jing Ma, Peter H. Gann, Frank M. Sacks, Lower prostate cancer risk in men with elevated plasma lycopene levels: results of a prospective analysis. Cancer Research. ,vol. 59, pp. 1225- 1230 ,(1999)
Jessica K. Campbell, Nancy J. Engelmann, Mary Ann Lila, John W. Erdman, Phytoene, Phytofluene, and Lycopene from Tomato Powder Differentially Accumulate in Tissues of Male Fisher 344 Rats Nutrition Research. ,vol. 27, pp. 794- 801 ,(2007) , 10.1016/J.NUTRES.2007.09.015
María Jesús Periago, Francisco Rincón, Maria Dolores Agüera, Gaspar Ros, Mixture Approach for Optimizing Lycopene Extraction from Tomato and Tomato Products Journal of Agricultural and Food Chemistry. ,vol. 52, pp. 5796- 5802 ,(2004) , 10.1021/JF049345H
María Jesús Periago, Francisco Rincón, Karin Jacob, Javier García-Alonso, Gaspar Ros, Detection of Key Factors in the Extraction and Quantification of Lycopene from Tomato and Tomato Products Journal of Agricultural and Food Chemistry. ,vol. 55, pp. 8825- 8829 ,(2007) , 10.1021/JF0705623
A. I. Khuri, J. A. Cornell, Response surfaces : designs and analyses ,(1987)
Devinder Kaur, Ali Abas Wani, DPS Oberoi, DS Sogi, None, Effect of extraction conditions on lycopene extractions from tomato processing waste skin using response surface methodology Food Chemistry. ,vol. 108, pp. 711- 718 ,(2008) , 10.1016/J.FOODCHEM.2007.11.002
Jessica K. Campbell, Randy B. Rogers, Mary Ann Lila, John W. Erdman, Biosynthesis of 14C-phytoene from tomato cell suspension cultures (Lycopersicon esculentum) for utilization in prostate cancer cell culture studies. Journal of Agricultural and Food Chemistry. ,vol. 54, pp. 747- 755 ,(2006) , 10.1021/JF0581269