α‐Luminol prevents decreases in glutamate, glutathione, and glutamine synthetase in the retinas of glaucomatous DBA/2J mice

摘要: Objective  To test the hypothesis that in DBA/2J mice, oxidative stress decreases glutamine synthetase (GS) levels resulting a loss of neuronal glutamate and antioxidant α-luminol (GVT®) this some types glaucoma. Animals  mice were separated into two groups, which one was not treated, other treated with GVT drinking water. At 7 months age, retinas examined from five untreated seven GVT-treated C57BL/6 (negative controls). Methods  Serial 0.5 μm plastic sections immunogold stained for glutamate, GS, total glutathione, followed by image analysis staining patterns density. Results  Focal immunostaining common inner nuclear layer (INL) retinas, but or retinas. Decreases glutathione GS found retinal regions where reduced. Retinas had no significant INL GS. Conclusions  dogs primary glaucoma are reported to have focal depletion glutamate. In similar changes occur prior development clinical disease. these glutamate-depleted regions, also reduced, consistent contributes glaucoma. The ability GVT, an antioxidant, inhibit abnormalities provides further support hypothesis.