MicroRNA hsa-let-7e-5p as a potential prognosis marker for rectal carcinoma with liver metastases.

摘要: MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression that target mRNAs for translational repression or cleavage. The present study was conducted to identify differentially expressed miRNAs in primary tumor tissues rectal carcinoma (RC) may be associated with heterochrony hepatic metastasis (HHM). Samples were collected exclusively from patients RC but not colon cancer (CC); Next-generation high-throughput sequencing technology and bioinformatics tools used profile analyze small RNAs their corresponding targets HHM (n=2) without metastases (non-metastatic, NM; n=2). A total 24 known identified (P<0.01; absolute value log2-fold change ≥1). Hsa-let-7e-5p exhibited the most significant elevation (log2-fold change=2.62). By combining online informatics resources previous mRNA data, it 54 validated genes let-7e downregulated HHM. number these have been demonstrated directly involved (including MYC proto-oncogene, bHLH transcription factor, high-mobility group AT-Hook 2, peptidase inhibitor 3, KIT proto-oncogene receptor tyrosine kinase, Jun AP-1 factor subunit ribonuclease T2), physiological associations immunity C-C motif chemokine 4 cluster differentiation 40 ligand) cellular metabolism peroxisome proliferator-activated γ, coactivator 1 α). Next, 14 selected reverse transcription-quantitative polymerase chain reaction analysis non-sequenced samples, downregulation 10 samples confirmed. In addition, hsa-let-7e-5p stimulated colorectal cell migration vitro. miRNA serve as a potential biomarker carcinoma-associated HHM, facilitating identification who at risk developing