Purification and characterization of ostrich prothrombin.
作者： Carminita Frost , Ryno Naudé , Willem Oelofsen , Koji Muramoto , Takako Naganuma
DOI: 10.1016/S1357-2725(00)00062-5
关键词:
摘要: Abstract The work focused on the penultimate enzyme, prothrombin, in coagulation cascade. Prothrombin was purified and characterized from ostrich plasma. results obtained contribute to a better understanding of blood evolution prothrombin plasma by barium chloride precipitation, ammonium sulfate fractionation, DEAE-cellulose Cu2+-chelate Sepharose chromatography. Ostrich exhibited Mr 72 800 pI 6.9 using SDS-PAGE PAG-isoelectrofocusing, respectively. N-terminal sequence showed 78 87% identity with human bovine, cDNA isolated liver predicted amino acid compared those other species. shares chicken (84%), (60%), bovine (59%), rat mouse (59%) hagfish (50%) suggesting common function these vertebrates. Amino identities indicate that thrombin β-chain (62%) propeptide-Gla (75%) domains are regions most constrained for functions vertebrate prothrombins. therefore, shows similarity structure
elsevier.com
cabdirect.org
sci-hub.se 参考文章(34)
M. Peter Esnouf, Peter H. Lloyd, Jolyon Jesty, A method for the simultaneous isolation of factor X and prothrombin from bovine plasma. Biochemical Journal. ,vol. 131, pp. 781- 789 ,(1973) , 10.1042/BJ1310781
Patthy L, Evolution of blood coagulation and fibrinolysis. Blood Coagulation & Fibrinolysis. ,vol. 1, pp. 153- ,(1990)
Russell F Doolittle, The evolution of vertebrate blood coagulation : a case of yin and yang Thrombosis and Haemostasis. ,vol. 70, pp. 24- 28 ,(1993) , 10.1055/S-0038-1646154
Gary L. Nelsestuen, Vitamin K-dependent plasma proteins Methods in Enzymology. ,vol. 107, pp. 507- 516 ,(1984) , 10.1016/0076-6879(84)07035-X
Paul Matejtschuk, Affinity separations: a practical approach IRL Press at Oxford University Press. ,(1997)
Ockert Tobias De Villiers, The blood of the ostrich University of South Africa. ,(1950)
C.M. Heldebrant, R.J. Butkowski, S.P. Bajaj, K.G. Mann, The Activation of Prothrombin II. PARTIAL REACTIONS, PHYSICAL AND CHEMICAL CHARACTERIZATION OF THE INTERMEDIATES OF ACTIVATION Journal of Biological Chemistry. ,vol. 248, pp. 7149- 7163 ,(1973) , 10.1016/S0021-9258(19)43373-5
L. Aurell, P. Friberger, G. Karlsson, G. Claeson, A new sensitive and highly specific chromogenic peptide substrate for Factor Xa Thrombosis Research. ,vol. 11, pp. 595- 609 ,(1977) , 10.1016/0049-3848(77)90018-4
Q. D. Dang, E. Di Cera, Residue 225 determines the Na(+)-induced allosteric regulation of catalytic activity in serine proteases Proceedings of the National Academy of Sciences of the United States of America. ,vol. 93, pp. 10653- 10656 ,(1996) , 10.1073/PNAS.93.20.10653
A.C.W. Swart, B.H.M. Kop-Klaassen, H.C. Hemker, Differential interaction of clotting factors II, VII, IX and X with sephadex and dextran blue. Pathophysiology of Haemostasis and Thrombosis. ,vol. 1, pp. 237- 252 ,(1972) , 10.1159/000213992